反应 #96221

反应方程式

ADP

glucose

→

ATP

溶剂

water

反应条件

后处理

1. 1
   其他The resting cells mentioned above were dried at a room temperature
2. 2
   其他dried further in a phosphorus pentoxide desiccator under a vacuum overnight
3. 3
   温度heated
4. 4
   其他for 3 minutes

实验过程

The resting cells mentioned above were dried at a room temperature and dried further in a phosphorus pentoxide desiccator under a vacuum overnight. Fifty mg of the dried cells were put into 2 ml of a reaction mixture containing 10 μmoles ADP, 200 μmoles glucose 250 μmoles phosphate buffer, 5 μmoles MgCl2.6H2O and the amount of the inhibitor shown in Table 5 or 6. The reaction mixture was held at 30° C. for 3 hours, and then heated in boiling water for 3 minutes. ATP formed in the reaction mixture was determined colorimetrically.

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