反应 #8486

反应方程式

cystine

O-methylisourea hydrogen sulfate

ammonium hydroxide

→

Guanidino-L-cystine

反应条件

后处理

1. 1
   其他The vial was sealed
2. 2
   温度The solution was then cooled
3. 3
   其他the ammonium hydroxide was removed by rotary evaporation
4. 4
   workup.DISSOLUTIONThe solid was then dissolved in water (20 mL)
5. 5
   过滤filtered through a cotton plug
6. 6
   其他The product was then isolated by ion exchange chromatography
7. 7
   洗涤eluting with hydrochloric acid (100 mM)

实验过程

To a solution of cystine (1 gm, 4.2 mmol) in ammonium hydroxide (10 mL) in a screw-capped vial was added O-methylisourea hydrogen sulfate (1.8 gm, 10 mmol). The vial was sealed and heated to 60° C. for 16 h. The solution was then cooled and the ammonium hydroxide was removed by rotary evaporation. The solid was then dissolved in water (20 mL), filtered through a cotton plug. The product was then isolated by ion exchange chromatography using Bio-Rex 70 resin and eluting with hydrochloric acid (100 mM).

来源