反应 #833537

ord-b2f7a63f9a06431dac68f45da5d4ecf1

反应方程式

NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
→
CC(C)=CCC/C(C)=C/CC/C(C)=C/CC/C=C(\C)CC/C=C(\C)CCC=C(C)C
MF59

反应物

—
MF59-0
NC(CO)(CO)CO.O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate Tris
—
2
O=P([O-])(O)O.O=P([O-])([O-])O.[Cl-].[Cl-].[K+].[K+].[Na+].[Na+].[Na+]
phosphate buffered saline
—
MF59-0

试剂

无

反应条件

详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    其他to yield a total of 0.7 ml
  2. 2
    workup.ADDITIONThe composition was gently mixed
  3. 3
    其他produced in Example 1
  4. 4
    其他to yield a total of 3 ml
  5. 5
    workup.ADDITIONThe composition was gently mixed

实验过程

10 Baboons were divided into two groups (five baboons per group) and administered the formulations specified in Table 1. For Group 1, equal parts of the adjuvant MF59-0, and p24gag/sf2 (in a citrate/Tris buffer) were combined to yield a total of 0.7 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected intramuscularly (IM) in the thigh muscle. For Group 2, 1.5 ml of 2× phosphate buffered saline (PBS) was added to 46.8 mg of the PLG-entrapped p24gag/sf2 formulation produced in Example 1. The material was vortexed for about 30 seconds until all beads were in suspension. 1.5 ml of MF59-0 was added to the resuspended beads to yield a total of 3 ml. The composition was gently mixed and 500 μl (to yield 100 μg p24gag/sf2/dose) of vaccine was injected IM in the thigh muscle.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US07393630B2uspto-grants-2008_07