反应 #68769

ord-28b9f6fe397143c3afbbfc2187eacc8c

反应方程式

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
COc1ccc(-c2oc3c(CC=C(C)C)c(O)cc(O)c3c(=O)c2O[C@@H]2O[C@H](C)[C@@H](O)[C@H](O)[C@@H]2O)cc1
icariside II

反应物

COc1ccc(-c2oc3c(CC=C(C)C)c(O[C@@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)cc(O)c3c(=O)c2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)cc1
icariin
O=P([O-])(O)O.O=P([O-])([O-])O.[Na+].[Na+].[Na+]
sodium dihydrogen phosphate disodium hydrogen phosphate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose

试剂

无

溶剂

—
β

反应条件

温度
40°CELSIUS
详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    其他to remove glucoside
  2. 2
    温度Then stop heating
  3. 3
    萃取repeatedly extract with ethyl acetate
  4. 4
    其他dried
  5. 5
    萃取Then repeatedly extract with methanol

实验过程

Fifty grams icariin were added into sodium dihydrogen phosphate-disodium hydrogen phosphate buffer (pH5.5) and 100 ml β glucose. 100 ml of β glucosidase was added and the solution was heated to 40° C. with stirring for 24 hours in order to remove glucoside. Then stop heating and repeatedly extract with ethyl acetate. The ethyl acetate layer was pooled and dried. Then repeatedly extract with methanol. The HPLC analysis confirmed that the content of pure icariside II 50.1%. Thus extract comprising icariside II is obtained.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US08530433B2uspto-grants-2013_09