反应 #68601

反应方程式

sodium hydrosulfide

tert-butyl (6-(4-methyl-3-oxopent-1-ynyl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methylcarbamate

hydroxylamine-O-sulfonic acid

sodium bicarbonate

→

tert-butyl (6-(3-isopropylisothiazol-5-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methylcarbamate

收率 22.5%

溶剂

water

water

1,4-dioxane

反应条件

后处理

1. 1
   workup.STIRRINGThe reaction was stirred at room temperature overnight
2. 2
   萃取extracted with DCM (40 mL)
3. 3
   干燥dried over sodium sulfate
4. 4
   过滤filtered
5. 5
   浓缩concentrated in vacuo
6. 6
   浓缩The concentrated crude material
7. 7
   workup.DISSOLUTIONwas dissolved in minimal DCM
8. 8
   其他was purified via MPLC (eluting with 0-10% MeOH/NH4OH in DCM)

实验过程

An aqueous mixture of tert-butyl (6-(4-methyl-3-oxopent-1-ynyl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methylcarbamate (0.800 g, 2.33 mmol) and hydroxylamine-O-sulfonic acid (0.263 g, 2.33 mol) in water (8.0 mL) and 1,4-dioxane (8.0 mL) was stirred at 0° C. until complete consumption of the organic starting material was observed. The mixture was then carefully treated with solid sodium bicarbonate (0.196 g, 2.33 mmol), followed by treatment with 1.4 M aqueous sodium hydrosulfide (1.83 ml, 2.56 mmol). The reaction was stirred at room temperature overnight. The reaction mixture was diluted with water (20 mL) and extracted with DCM (40 mL), dried over sodium sulfate, filtered and concentrated in vacuo. The concentrated crude material was dissolved in minimal DCM and was purified via MPLC (eluting with 0-10% MeOH/NH4OH in DCM) to yield tert-butyl (6-(3-isopropylisothiazol-5-yl)-[1,2,4]triazolo[4,3-b]pyridazin-3-yl)methylcarbamate (0.196 g, 22.5% yield) with 82% purity. MS (ESI pos. ion) m/z: 433.0 (M+H). Calc'd Exact Mass for C21H20N8OS: 432.51.

来源