反应 #6711

反应方程式

triethylamine

Z-D-Pro-OH

H-Val-MeVal-Pro-Pro-NHBn

HCl

→

Z-D-Pro-Val-MeVal-Pro-Pro-NHBn

溶剂

DMF

反应条件

后处理

1. 1
   其他the DMF was evaporated under reduced pressure
2. 2
   workup.ADDITIONThe residue was diluted with ethyl acetate
3. 3
   洗涤thoroughly washed with dilute aqueous HCl (pH 2), water, dilute aqueous NaOH (pH 9–10), and water
4. 4
   干燥The organic phase was dried over sodium sulfate
5. 5
   其他evaporated to dryness
6. 6
   其他to yield 9.2 g of the

实验过程

3.74 g Z-D-Pro-OH (15 mmol, BACHEM) and 8.25 g H-Val-MeVal-Pro-Pro-NHBn×HCl (15 mmol) were dissolved in 80 ml dry DMF. After cooling to 0° C., 2.4 g DEPCN (2.25 ml, 15 mmol) and 4.2 ml triethylamine (30 mmol) were added. The reaction mixture was stirred at 0° C. for several hours and room temperature overnight, then the DMF was evaporated under reduced pressure. The residue was diluted with ethyl acetate and thoroughly washed with dilute aqueous HCl (pH 2), water, dilute aqueous NaOH (pH 9–10), and water. The organic phase was dried over sodium sulfate and evaporated to dryness to yield 9.2 g of the desired protected pentapeptide.

来源