反应 #554626

ord-2f76e5374e3a4cca9a75d25d4a1a627f

反应方程式

O=C[C@H](O)[C@@H](O)[C@H](O)CO
D-xylose
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
→
OC[C@@H](O)[C@H](O)[C@@H](O)CO
xylitol

反应物

O=C[C@H](O)[C@@H](O)[C@H](O)CO
D-xylose
—
5C
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
—
6B

试剂

无

溶剂

CCO
ethanol

反应条件

详细条件
See reaction.notes.procedure_details.

实验过程

After 25 hours, strain CD804 (Ex. 4D) had consumed 15 g/L D-xylose and produced about 4.7 g/L of xylitol and 1 g/L of ethanol from the flask containing the glucose isomerase. In contrast, strains CD805 (Ex. 5C) and CD806 (Ex. 6B) had each consumed 25 g/L D-xylose in the presence of glucose isomerase. Strain CD805 produced in this time about 1.9 g/L of xylitol and 7.1 g/L of ethanol. Strain CD806 produced in this time about 1.8 g/L of xylitol and 6.8 g/L of ethanol. Xylulose seems to be consumed by the strains at very high rates, something which is not observed in S. cerevisiae. The non-oxidative pentose phosphate pathway controls the fermentation rate of xylulose but not of xylose in S. cerevisiae TMB3001. FEM Yeast Res. 2002 August; 2(3):277-82. Johansson B, Hahn-Hagerdal B). Without added glucose isomerase, each of strains CD804, CD805 and CD806 consumed xylose very slowly.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US08623633B2uspto-grants-2014_01