反应 #498288

反应方程式

glycine

p-nitrophenyl-β-D-cellobioside

gluconolactone

sodium acetic acid

→

p-nitrophenol

反应条件

后处理

1. 1
   其他an incubation of 15 minutes
2. 2
   其他at 30, 40, 50, 60 or 70° C.

实验过程

To determine the influence of the temperature on the enzyme activity 25 μl enzyme solution was added to 225 μl of a solution containing 0.1% p-nitrophenyl-β-D-cellobioside and 25 mM gluconolactone in a 50 mM sodium acetic acid buffer at pH 5.0. Following an incubation of 15 minutes at 30, 40, 50, 60 or 70° C. the enzyme activity was stopped by adding 750 μl of a 0.5 M glycine buffer pH 9.0. The concentration of p-nitrophenol formed was determined spectrophotometrically at 400 nm.

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