反应 #465930

反应方程式

p-phenylenediamine

DI water

→

1-naphthol

溶剂

CH3COONa

反应条件

后处理

1. 1
   workup.ADDITION100 ml “A” was added to one beaker and 50 ml “A” and 50 ml “B”
2. 2
   其他to form 100 ml in a second beaker
3. 3
   其他The LOM beakers were sealed
4. 4
   workup.ADDITIONThe spent liquor was poured off
5. 5
   洗涤the swatches were rinsed in cold tap water for about 15 minutes
6. 6
   其他The swatches were dried at room temperature

实验过程

A 0.5 mg/ml solution of a first compound (p-phenylenediamine, “A”) and a 0.5 mg/ml solution of a second compound (1-naphthol, “B”) was prepared by dissolving the compound in the appropriate amount of 0.1 M CH3COONa, pH 5.5, buffer. A total volume of 100 ml was used in each LOM beaker. 100 ml “A” was added to one beaker and 50 ml “A” and 50 ml “B” were combined to form 100 ml in a second beaker. Swatches of the materials listed above were wetted in DI water and soaked in the precursor solutions. A Myceliophthora thermophila laccase (“MtL”) with an activity of 690 LACU/ml (80 LACU/mg) was added to each beaker at a concentration of 12.5 mg/I. The LOM beakers were sealed and mounted in the LOM. After 1 hour at 42 RPM and 30° C., the LOM was stopped. The spent liquor was poured off and the swatches were rinsed in cold tap water for about 15 minutes. The swatches were dried at room temperature and CIELAB values were measured for all of the swatches using the Macbeth ColorEye 7000. The results are given in Tables 21 and 22.

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