反应 #454165

ord-6d54da814d2d4eabac67e5fa5a5b25b8

反应方程式

CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
O=C(O)/C=C\C(=O)O
maleic acid
O=C(O)/C=C\C(=O)O
maleic acid
→
O=C(O)/C=C/C(=O)O
fumaric acid
O=C(O)/C=C\C(=O)O
maleic acid

反应物

CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O
ampicillin
O=C(O)/C=C\C(=O)O
maleic acid
O=C(O)/C=C\C(=O)O
maleic acid

试剂

[Pt]
platinum

反应条件

详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    其他the microorganism was recovered
  2. 2
    其他reacted at 30° C. overnight
  3. 3
    其他prepared from E

实验过程

The ampicillin-resistant transformant was selected by measuring its enzyme activity of isomerizing maleic acid. The ampicillin-resistant transformant was inoculated via a loop of platinum into 3 ml maleic acid medium (Table 1) containing 100 γ ampicillin and cultured overnight, and the microorganism was recovered by centrifuging 1.5 ml of the culture. The recovered microorganism was suspended in 1 ml of 20% maleic acid substrate solution (Table 2) and reacted at 30° C. overnight. This substrate solution contained aspartase prepared from E. coli so that fumaric acid formed by isomerization of maleic acid was converted into L-aspartic acid. After overnight reaction, a part of the reaction solution was diluted 400-fold with distilled water and measured for its formed L-aspartic acid by HPLC analysis. The conditions for HPLC analysis were as described in item (2) in Example 1.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US06133014uspto-grants-2000_10