反应 #417937

ord-0cfa3d0c62b9470ea603501e1e2e5dc9

反应方程式

NC(=O)CC[C@H](N)C(=O)O
glutamine
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[Na+]
penicillin G sodium
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]3N=C(N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O.O=S(=O)(O)O
streptomycin sulfate
CN[C@@H]1[C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@H]([C@@H](C)NC)CC[C@H]3N)[C@@H](N)C[C@H]2N)OC[C@]1(C)O
gentamicin
→
O=C([O-])O.[Na+]
sodium bicarbonate
收率 0.1%
O=S(=O)(O)CCN1CCN(CCO)CC1
HEPES

反应物

NC(=O)CC[C@H](N)C(=O)O
glutamine
CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(=O)[O-].[Na+]
penicillin G sodium
CN[C@@H]1[C@H](O[C@H]2[C@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](N=C(N)N)[C@@H](O)[C@@H]3N=C(N)N)O[C@@H](C)[C@]2(O)C=O)O[C@@H](CO)[C@H](O)[C@H]1O.O=S(=O)(O)O
streptomycin sulfate
CN[C@@H]1[C@@H](O)[C@@H](O[C@@H]2[C@@H](O)[C@H](O[C@H]3O[C@H]([C@@H](C)NC)CC[C@H]3N)[C@@H](N)C[C@H]2N)OC[C@]1(C)O
gentamicin

试剂

无

反应条件

详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    温度Human MCF-7 breast carcinoma cells were maintained in RPMI 1640 medium

实验过程

Human MCF-7 breast carcinoma cells were maintained in RPMI 1640 medium containing 100 units/mL penicillin G sodium, 100 μg/mL streptomycin sulfate, and 25 μg/mL gentamicin. The medium was supplemented with 10% heat-inactivated fetal bovine serum and 2 mM glutamine. Additional buffering was provided with 10 mM HEPES and 0.075% sodium bicarbonate. The tumor cells were cultured in tissue culture flasks in a humidified incubator at 37° C. in an atmosphere of 95% air and 5% CO2.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US08877715B2uspto-grants-2014_11