反应 #342989

反应方程式

Camptothecin

glycolic acid

ammonium persulfate

→

7-hydroxymethylcamptothecin

溶剂

sulfuric acid

反应条件

后处理

1. 1
   workup.ADDITIONwas added dropwise
2. 2
   温度to cool
3. 3
   萃取was then extracted with chloroform (100 ml×3)
4. 4
   洗涤washed with a 7% aqueous solution of sodium bicarbonate (300 ml)
5. 5
   干燥with a saturated edible salt solution (100 ml), dried with anhydrous magnesium sulfate
6. 6
   过滤filtered
7. 7
   其他evaporated until dryness under reduced pressure
8. 8
   其他separation and purification of 7-hydroxymethylcamptothecin whereupon 10.3 mg (19.0%) of pure 7-hydroxymethylcamptothecin
9. 9
   其他were obtained
10. 10
    其他Besides this, 10.7 mg of camptothecin were recovered

实验过程

Camptothecin (50 mg, 0.143 m-mol) was dissolved in 75% sulfuric acid (3 ml). To this solution were added glycolic acid (500 mg, 6.57 m-mol) and silver nitrate (250 mg, 1.31 m-mol) and then was added dropwise under heating (100°-110° C.) and agitation and aqueous solution (15 ml) of ammonium persulfate (3.00 g, 0.0131 m-mol) over 2 hours. After the reaction mixture was allowed to cool, ice water (100 ml) was poured into the reaction mixture which was then extracted with chloroform (100 ml×3). The chloroform layers were combined, washed with a 7% aqueous solution of sodium bicarbonate (300 ml) and then with a saturated edible salt solution (100 ml), dried with anhydrous magnesium sulfate, filtered and evaporated until dryness under reduced pressure. The residue was subjected to thin layer chromatography (5% methanol-chloroform) to effect separation and purification of 7-hydroxymethylcamptothecin whereupon 10.3 mg (19.0%) of pure 7-hydroxymethylcamptothecin were obtained. Besides this, 10.7 mg of camptothecin were recovered.

来源