反应 #324426

反应方程式

HCl methanol

Pulchinenoside A

→

desired product

收率 85.0%

3,23-Dihydroxy-20(29)-lupen-28-oic acid

收率 85.0%

溶剂

water

反应条件

后处理

1. 1
   萃取the mixture was extracted with chloroform (3×50 ml)
2. 2
   洗涤The combined organic layer was washed with H2O (1×10 ml), brine (1×10 ml)
3. 3
   干燥dried over sodium sulfate
4. 4
   浓缩concentrated under vacuum
5. 5
   其他The resulting residue was purified by column chromatography on silica gel

实验过程

To a solution of HCl methanol reagent 10 (10 mL) was added Pulchinenoside A (DA001, 100 mg) and the resulting mixture was stirred at 35° C. for 4 days. After pouring into water (10 ml), the mixture was extracted with chloroform (3×50 ml). The combined organic layer was washed with H2O (1×10 ml), brine (1×10 ml), dried over sodium sulfate and concentrated under vacuum. The resulting residue was purified by column chromatography on silica gel using CH2Cl2-MeOH (v/v, 30:1) to yield the desired product as a white solid with 85% yield.

来源