反应 #2777

ord-989c68d91dfc4d74a9b62cfdff0165f9

反应方程式

O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21.O=C1CCC(=O)N1O
biotin-N-hydroxysuccinimide
NCCc1ccc(O)cc1
tyramine
CC[NH+](CC)CC.O=C([O-])O
triethylammonium bicarbonate
→
NCCc1ccc(O)cc1.O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21
biotin tyramine

反应物

O=C(O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@@H]21.O=C1CCC(=O)N1O
biotin-N-hydroxysuccinimide
NCCc1ccc(O)cc1
tyramine
CC[NH+](CC)CC.O=C([O-])O
triethylammonium bicarbonate

试剂

无

溶剂

CN(C)C=O
dimethylformamide

反应条件

温度
50°CELSIUS
详细条件
See reaction.notes.procedure_details.

实验过程

a solution of biotin-N-hydroxysuccinimide, 170 mg (0.5 mMoles), and tyramine (recrystallized from water), 68.5 mg (0.5 mMoles), in 25 ml dimethylformamide was treated with 10 ml of 1M triethylammonium bicarbonate, pH 7.5, and then heated at 50° C. for 3 hours.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US05731158uspto-grants-1998_03