反应 #2507845

ord-6c8e633799a94f37a39153d5f8c3caac

反应方程式

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl
→
OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
Trehalose

反应物

O.O.OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
trehalose dihydrate
CC(C[*:1])[*:2]
polypropylene
Cl
HCl

试剂

无

溶剂

O
water
O
water

反应条件

详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    workup.ADDITIONwas added at room temperature
  2. 2
    其他was obtained
  3. 3
    其他to obtain 400 ml final volume
  4. 4
    过滤The solution was filtered through a 0.22-micron
  5. 5
    过滤filter
  6. 6
    workup.ADDITIONto dilute the protein solution

实验过程

25.48 g of trehalose dihydrate was carefully weighed and transferred into a sterile polypropylene bottle, to which 350 ml of purified water was added at room temperature and stirred slowly until a clear solution was obtained. To the clear solution, 0.1N HCl was added drop by drop to adjust the pH to 3.9, then the volume was adjusted with purified water to obtain 400 ml final volume. The pH was measured and found to be 4.2. The solution was filtered through a 0.22-micron filter and was used directly to dilute the protein solution.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US07956028B2uspto-grants-2011_06