反应 #2314333

ord-ba6c176d40b84966a7c2f1ff237718fc

反应方程式

Nc1ncc(O)c(N)n1
CDKi
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione
→
Nc1ncc(O)c(N)n1
CDKi
N[C@@H](Cc1c[nH]cn1)C(=O)O
histidine

反应物

Nc1ncc(O)c(N)n1
CDKi
N[C@@H](CCC(=O)N[C@@H](CS)C(=O)NCC(=O)O)C(=O)O
glutathione

试剂

无

反应条件

详细条件
See reaction.notes.procedure_details.

实验过程

W3, W8, and W10 were further subcloned into a modified pGEX4T-1 plasmid (Pharmacia Biotech, Uppsala, Sweden) (where a NdeI cloning site was inserted between the BamHI and EcoRI sites) as NdeI-NotI fragments to generate glutathione S-transferase (GST) tagged fusion proteins. A similar strategy was used to generate fusion proteins without the (Gly4Ser)31inker (i.e., W4 (p27-p16), W7 (p2712-178-p16 fusion CDKi W9 (p2725-93-p16)). The nucleic acid and amino acid sequences of the p2725-93-p16 fusion CDKi, W9, without the HA tag and six histidine residues are provided in SEQ ID NO: 23 and SEQ ID NO: 24, respectively.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US06413943B1uspto-grants-2002_07