反应 #2314320

ord-beb24972460942a1a329220025e9ae3f

反应方程式

O=C(O)[C@@H]1CCCN1
L-proline
O=C(O)CCC(=O)C(=O)O
2-ketoglutaric acid
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
O=S(=O)(O)CCNC(CO)(CO)CO
TES
→
O=C(O)[C@H]1NCC[C@H]1O
cis-3-hydroxy-L-proline

反应物

O=C(O)[C@@H]1CCCN1
L-proline
O=C(O)CCC(=O)C(=O)O
2-ketoglutaric acid
O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
L-ascorbic acid
—
ferrous sulfate
O=S(=O)(O)CCNC(CO)(CO)CO
TES

试剂

无

反应条件

温度
100°CELSIUS
详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    其他The cells, the treated cells or the enzyme preparation
  2. 2
    其他the reaction

实验过程

The cells, the treated cells or the enzyme preparation are added to 240 mM TES [N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid] buffer containing 12 mM L-proline, 24 mM 2-ketoglutaric acid, 4 mM ferrous sulfate and 8 mM L-ascorbic acid to make 250 μl in total. The mixture is kept at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by HPLC.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: US06413748B1uspto-grants-2002_07