反应 #2314319

反应方程式

L-proline

2-ketoglutaric acid

L-ascorbic acid

→

cis-3-hydroxy-L-proline

反应条件

后处理

1. 1
   其他The enzyme preparation
2. 2
   workup.ADDITIONto be determined is added to 100 mM TES buffer (pH 7.0)
3. 3
   其他the reaction

实验过程

The enzyme preparation to be determined is added to 100 mM TES buffer (pH 7.0) containing 5 mM L-proline, 5 mM 2-ketoglutaric acid, 1 mM ferrous sulfate and 5 mM L-ascorbic acid to make 100 μl in total, and the mixture was allowed to stand at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes so as to stop the reaction, and the amount of cis-3-hydroxy-L-proline produced in the reaction mixture is determined by high performance liquid chromatography (hereinafter referred to as HPLC).

来源