反应 #2200578
ord-22dfff47d7d344e4866063dcc9b46fe2
反应方程式
sodium benzoate
citric acid
vanillic acid
→
guaiacol
反应条件
详细条件
See reaction.notes.procedure_details.
后处理
- 1workup.ADDITIONadded
- 2workup.ADDITIONtwo steps of preparations containing 103 and 105 spores/ml of A
- 3其他acidoterrestris spores were prepared
- 4其他cultured at 45° C.
- 5其他As a control, a sample prepared
- 6workup.ALIQUOTThe resulting solution was sampled with a lapse of time
实验过程
Using A. acidoterrestris-proliferative acid sport drink (comprising 1% fruit juice, 0.18% citric acid, 9% isomerized sugar and 0.25% flavor) containing 100 ppm of vanillic acid added therein, two steps of preparations containing 103 and 105 spores/ml of A. acidoterrestris spores were prepared therein and cultured at 45° C. with shaking. As a control, a sample prepared by adding 300 ppm of sodium benzoate having the effect of inhibiting the growth of the same bacteria to the same solution was used. The resulting solution was sampled with a lapse of time and the amount of guaiacol produced was measured by the Peroxidase method.