反应 #1860184

反应方程式

1,2,3,4-tetrafluoro-5,8-dihydroxyanthraquinone

2-Amino-5-diethylaminopentane

→

1,4-bis(5-(diethylamino)pentan-2-ylamino)-2,3-difluoro-5,8-dihydroxyanthracene-9,10-dione

溶剂

CH2Cl2

反应条件

后处理

1. 1
   其他After evaporation of the solvents
2. 2
   其他the residue was purified by silica gel chromatography
3. 3
   其他yielding 364 mgs of Dye 2 as dark blue product

实验过程

A mixture of 1,2,3,4-tetrafluoro-5,8-dihydroxyanthraquinone (0.47 g, 1.5 mmol) and 2-Amino-5-diethylaminopentane (2.3 mL) in CH2Cl2 (20 mL) was stirred at room temperature for 12 hours. After evaporation of the solvents, the residue was purified by silica gel chromatography using isocratic solvent system of CH2Cl2/MeOH/Et3N (100:10:1) yielding 364 mgs of Dye 2 as dark blue product. Abs (max, PBS pH 7.4)=667 nm; Em=700 nm. The structure of Dye 2 is given below:

来源