反应 #1824350

反应方程式

L-proline

2-ketoglutarate

→

cis-3-hydroxy-L-proline

反应条件

后处理

1. 1
   其他The enzyme reaction
2. 2
   其他using purified L-proline-3-hydroxylase
3. 3
   其他obtained in Example 5
4. 4
   其他As a result of the reaction

实验过程

The enzyme reaction using purified L-proline-3-hydroxylase obtained in Example 5 was carried out. The reaction mixture was composed of 200 mM TES buffer (pH 7.0), 20 mM L-proline, 20 mM 2-ketoglutarate, 5 mM L-ascorbic acids 1 mM, ferrous sulfate, and 106 U of purified enzyme preparation, the total volume being 100 μl. The reaction was carried out at 35° C. for 30 minutes. As a result of the reaction, 18 mM (2.4 g/l) of cis-3-hydroxy-L-proline was produced in the reaction mixture.

来源