反应 #1747849
ord-34cbc220044e4ddfa08a0e8d4e75a805
反应方程式
反应条件
后处理
- 1其他to react for at least 24 hours at room temperature
- 2其他To separate fluorescently labeled desferrioxamine from free fluorescein and free desferrioxamine
- 3其他the mixture is separated
- 4洗涤are eluted in 2 mL fractions
- 5洗涤The fluorescein-desferrioxamine is eluted in the void volume of the column
- 6其他The first peak is collected
- 7其他used for preparing fluorescein-desferrioxamine, desferrioxamine, and rhodamine liposomes
实验过程
An aliquot of 120 mg of desferrioxamine and 233.64 mg of fluorescein isothiocyanate (FITC) are mixed in 50 mL of 0.1 M sodium carbonate pH 9.5. A molar ratio of 3:1 of FITC: desferrioxamine is used as described in Hentz et al. (1997) Anal. Chem. 69:4994-5000. The reaction mixture is wrapped in aluminum foil to avoid light exposure and allowed to react for at least 24 hours at room temperature. To separate fluorescently labeled desferrioxamine from free fluorescein and free desferrioxamine, the mixture is separated using a P-2 column (Bio-Rad) equilibrated in saline and samples are eluted in 2 mL fractions. The fluorescein-desferrioxamine is eluted in the void volume of the column. The first peak is collected and used for preparing fluorescein-desferrioxamine, desferrioxamine, and rhodamine liposomes.