反应 #1747848

反应方程式

DOPA

methanol

L-DOPA

methanol

methyl trifluoroacetate

→

N-trifluoroacetyl-L-DOPA

溶剂

water

反应条件

后处理

1. 1
   其他equipped with a Teflon stirring bar
2. 2
   其他rubber septum and purged with dry argon
3. 3
   workup.DISSOLUTIONhad completely dissolved (after about 24 hr)
4. 4
   过滤The mixture was filtered
5. 5
   其他The filtrate was evaporated under reduced pressure
6. 6
   其他The crude product was re-crystallized from ethyl acetate
7. 7
   其他Use of ester of N-hydroxysuccinimide in the synthesis of N-acrylamino acids

实验过程

1.97 g L-DOPA (10 mmol) and 5 ml of dry methanol was added into an oven-dried 25 ml round-bottomed flask equipped with a Teflon stirring bar and rubber septum and purged with dry argon. Then, 1.3 ml of TMG (10 mmol) and 1.4 ml of methyl trifluoroacetate (12.5 mmol) was added. The mixture was stirred vigorously at room temperature until DOPA had completely dissolved (after about 24 hr). At this point, the flask was immersed in cold water (0° C.) and 5 ml of methanol and 4 g Dowex 50 resin (H+ form) were added. The mixture was filtered after stirring for 10 min. The filtrate was evaporated under reduced pressure. The crude product was re-crystallized from ethyl acetate and the yield of N-trifluoroacetyl-L-DOPA was 78%. See Curphey, T. J. Trifluoroacetylation of amino acids and peptides by ethyl trifluoroacetate. J. Org. Chem. 1979, 44, 2805-2807; Lapidot, Y.; Rappoport, S.; Wolman, Y. Use of ester of N-hydroxysuccinimide in the synthesis of N-acrylamino acids. J. Lipid Research 1967, 8, 142-145.

来源