反应 #1706038
ord-b5c517e3171841e8b0d3159d073d3469
反应方程式
反应条件
后处理
- 1其他A 100 ml screw cap bottle
- 2其他was prepared
- 3其他was performed at 30° C.
- 4workup.ALIQUOTThe culture liquid was sampled periodically
- 5其他was used at 60° C. as the liquid chromatography column
- 6浓缩in the substrate concentrations and product concentrations in fermentation medium of each transformant
实验过程
A 100 ml screw cap bottle having an exhaust line with a check valve attached to the lid was used for the fermentation test. 50 ml of SDX medium (6.7 g/l yeast nitrogen base without amino acids and nucleic acids, 30 g/l D-glucose, 20 g/l xylose) with liquid yeast suspension added to a final OD600 of 10 of the fermentation medium was prepared, and fermentation was performed at 30° C., 100 rpm. The culture liquid was sampled periodically, and the substrate (glucose and xylose) and products (ethanol, glycerol and xylitol) were analyzed by liquid chromatography. An HPX-87H column (Bio-Rad) was used at 60° C. as the liquid chromatography column, and a RID-10A refractive index detector (Shimadzu Mfg.) was used as the detector. 0.05% sulfuric acid solution was used for the mobile phase, which was supplied at a rate of 0.6 ml/min. FIG. 12 shows changes over time in the substrate concentrations and product concentrations in fermentation medium of each transformant. The fermentation tests were performed twice or more, and the averages are given.