反应 #1702032

反应方程式

irinotecan hydrochloride trihydrate

sodium

cysteic acid

→

irinotecan

溶剂

Methanol

反应条件

后处理

1. 1
   其他evaporated in vacuo
2. 2
   workup.ADDITION100 ml of water was added to the residue
3. 3
   其他obtained
4. 4
   其他after evaporation
5. 5
   其他transparent (approx. 30 min)
6. 6
   过滤The obtained solution was filtered through 0.2 mm
7. 7
   过滤filter
8. 8
   其他freeze dried
9. 9
   过滤The filtration

实验过程

Methanol stock-solutions of irinotecan hydrochloride trihydrate (100 ml, 1.15 mg/ml) and sodium salt of methyl ester of N-all-trans-retinoyl cysteic acid (27 ml, 15 mg/ml) were mixed in a 500 ml round-bottom flask and evaporated in vacuo. 100 ml of water was added to the residue obtained after evaporation and the mixture was stirred until it became clear and transparent (approx. 30 min). The concentration of irinotecan in the obtained solution was 1 mg/ml, corresponding to an irinotecan hydrochloride trihydrate concentration of 1.15 mg/ml. The obtained solution was filtered through 0.2 mm filter and freeze dried. The filtration did not result in reduction of irinotecan concentration.

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