反应 #155358

反应方程式

D3

cholecalciferol

7-Dehydrocholesterol

cholecalciferol

25-Hydroxyvitamin D

7-DHC

7-DHC

→

7-DHC

溶剂

ethanol

ethanol

ethanol

反应条件

后处理

1. 1
   过滤filtered with a 0.22 mm pore size
2. 2
   过滤filter before use
3. 3
   其他Fourteen different groups were prepared
4. 4
   其他eight were non irradiated

实验过程

For the treatment with vitamin D metabolites, stock solutions of 2 mM 7-Dehydrocholesterol (7-DHC, Sigma St. Louis, Mo., USA), 2 μM cholecalciferol (D3, Sigma St. Louis, Mo., USA), and 2 μM 25-Hydroxyvitamin D (25-D3, Sigma St. Louis, Mo., USA) were prepared in absolute ethanol and filtered with a 0.22 mm pore size filter before use. Fourteen different groups were prepared; eight were non irradiated: 7-DHC at different doses (20 nmols, 2 nmols, 0.2 nmols, 2×10−2 nmols, and 2×10−3 nmols), 2×10−2 nmols D3, 2×10−2 nmols 25-D3 and ethanol. And six were UV-irradiated: 7-DHC at the same different doses (20 nmols, 2 nmols, 0.2 nmols, 2×10−2 nmols, and 2×10−3 nmols) and ethanol.

来源