反应 #1265349

反应方程式

histidine

sodium chloride

→

histidine sodium chloride

溶剂

water

反应条件

后处理

1. 1
   其他Ethanol was removed from the liposome suspension by diafiltration
2. 2
   过滤The solution was filtered through a 0.22 μm
3. 3
   过滤Durapore filter
4. 4
   workup.ADDITIONThe liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution
5. 5
   其他to remove the ethanol

实验过程

Ethanol was removed from the liposome suspension by diafiltration. A histidine/sodium chloride solution was prepared by dissolving histidine (10 mM) and sodium chloride (150 mM) in sterile water. The pH of the solution was adjusted to approximately 7. The solution was filtered through a 0.22 μm Durapore filter. The liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution and diafiltered through a polysulfone hollow-fiber ultrafilter. Eight volume exchanges were performed against the histidine/sodium chloride solution to remove the ethanol. The process fluid temperature was maintained at about 20-30° C. Total diafiltration time was approximately 4.5 hours.

来源