反应 #1244303

反应方程式

Ethyl (3-(N-(tert-butoxycarbonyl)amino)-1-fluoro-2-hydroxypropyl)(1,1-diethoxyethyl)phosphinate

Hydrochloric acid

→

(3-amino-1-fluoro-2-hydroxypropyl)phosphinic acid

收率 56.0%

溶剂

methanol

反应条件

后处理

1. 1
   其他sparged with argon immediately
2. 2
   浓缩The reaction mixture was concentrated under reduced pressure
3. 3
   workup.DISSOLUTIONthe crude product was re-dissolved in methanol (5 mL)
4. 4
   其他the residual water removed by co-evaporation under reduced pressure with methanol
5. 5
   其他The crude product (70 mg) was purified by column chromatography (1×10 cm column)
6. 6
   洗涤eluting with methylene chloride, methanol, concentrated ammonium hydroxide (6:3:1)
7. 7
   workup.ADDITIONThe fractions containing product
8. 8
   浓缩were concentrated under reduced pressure, co-evaporated with acetonitrile (2×10 ml)
9. 9
   干燥with methanol (1×10 mL) and dried overnight under high vacuum (0.1 mm Hg)

实验过程

Ethyl (3-(N-(tert-butoxycarbonyl)amino)-1-fluoro-2-hydroxypropyl)(1,1-diethoxyethyl)phosphinate (180 mg, 4.5 mmol) was dissolved in methanol (2 mL), treated with 3 N Hydrochloric acid (20 mL, 60.0 mmol, sparged with argon immediately prior to use). The mixture was stirred at room temperature for 6 hours under an argon atmosphere. The reaction mixture was concentrated under reduced pressure, the crude product was re-dissolved in methanol (5 mL); the residual water removed by co-evaporation under reduced pressure with methanol. The crude product (70 mg) was purified by column chromatography (1×10 cm column) eluting with methylene chloride, methanol, concentrated ammonium hydroxide (6:3:1). The fractions containing product were concentrated under reduced pressure, co-evaporated with acetonitrile (2×10 ml) then with methanol (1×10 mL) and dried overnight under high vacuum (0.1 mm Hg). This procedure afforded (3-amino-1-fluoro-2-hydroxypropyl)phosphinic acid as a white solid (40 mg, 56%). 1H NMR (300 MHz, D2O) δ 7.93 (s, 0.5H), 6.11 (s, 0.5 H), 4.60-4.20 (m, 2H), 3.42-3.08 (m, 2H).

来源