反应 #1244289

反应方程式

ethyl (S)-4-chloro-3-hydroxybutyrate

NaCN

→

ethyl (R)-4-cyano-3-hydroxybutyrate

溶剂

potassium phosphate

反应条件

后处理

1. 1
   洗涤After fifty seven hours the mixture was washed with ethyl acetate (2 times 250 mL)
2. 2
   干燥the combined organics dried over anhydrous sodium sulfate
3. 3
   过滤The mixture was filtered
4. 4
   其他evaporated on a rotary evaporator

实验过程

To a well stirred solution of ethyl (S)-4-chloro-3-hydroxybutyrate (8.25 g, 50 mmoles) in 300 mM potassium phosphate buffer, 300 mM NaCN pH 8.0 (1 L) at 30° C. was added halohydrin dehalogenase SEQ ID NO: 14 (9 g) as a lyophilized powder. After fifty seven hours the mixture was washed with ethyl acetate (2 times 250 mL) and the combined organics dried over anhydrous sodium sulfate. The mixture was filtered and evaporated on a rotary evaporator to give essentially pure ethyl (R)-4-cyano-3-hydroxybutyrate, as determined using the gas chromatography method and elution time data described in Example 6, hereinbelow.

来源