反应 #1244242

ord-a8157e506ba647088ae91dc2f0088eb6

反应方程式

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate
→
N[C@@H](CCC(=O)O)C(=O)O
L-glutamic Acid

反应物

O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-]
citrate
C=C(OP(=O)(O)O)C(=O)O
phosphoenolpyruvate
N[C@@H](CCC(=O)[O-])C(=O)[O-]
glutamate

试剂

无

反应条件

详细条件
See reaction.notes.procedure_details.

后处理

  1. 1
    workup.ADDITIONwere introduced into the SC17sucA strain

实验过程

Subsequently, the citrate synthase gene, phosphoenolpyruvate carboxylase gene and glutamate dehydrogenase gene derived from Escherichia coli were introduced into the SC17sucA strain.

来源

DOI: 10.6084/m9.figshare.5104873.v1专利: USRE041800E1uspto-grants-2010_10