Реакция #969876

Уравнение реакции

piperidine

Fmoc-Val-Pro doxorubicin

→

compound G

Выход 50.0%

H-Val-Pro doxorubicin

Выход 50.0%

Растворители

dimethylformamide

Условия реакции

Обработка

1. 1
   Другоеform red to purple)
2. 2
   ДругоеThen, the reaction mixture was evaporated to dryness under reduced pressure
3. 3
   Другоеthe residue thus obtained
4. 4
   Другоеwas purified by reverse phase chromatography with water/acetonitrile (70:1)

Методика

A solution of piperidine in dimethylformamide 50% (1.92 mL) was added to Fmoc-Val-Pro-doxorubicin (F) (29.7 mg, 0.03 mmol) and the reaction mixture was stirred at room temperature for 1 minute (the reaction colour changes form red to purple). Then, the reaction mixture was evaporated to dryness under reduced pressure and the residue thus obtained was purified by reverse phase chromatography with water/acetonitrile (70:1) to give the deprotected compound G (50% yield)

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