Реакция #927226

Уравнение реакции

histidine

sodium chloride

→

histidine sodium chloride

Растворители

water

Условия реакции

Обработка

1. 1
   ДругоеEthanol was removed from the liposome suspension by diafiltration
2. 2
   ФильтрацияThe solution was filtered through a 0.22 μm
3. 3
   ФильтрацияDurapore filter
4. 4
   workup.ADDITIONThe liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution
5. 5
   Другоеto remove the ethanol

Методика

Ethanol was removed from the liposome suspension by diafiltration. A histidine/sodium chloride solution was prepared by dissolving histidine (10 mM) and sodium chloride (150 mM) in sterile water. The pH of the solution was adjusted to approximately 7. The solution was filtered through a 0.22 μm Durapore filter. The liposome suspension was diluted in approximately a 1:1 (v/v) ratio with the histidine/sodium chloride solution and diafiltered through a polysulfone hollow-fiber ultrafilter. Eight volume exchanges were performed against the histidine/sodium chloride solution to remove the ethanol. The process fluid temperature was maintained at about 20-30° C. Total diafiltration time was approximately 4.5 hours.

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