Реакция #8956

Уравнение реакции

L-lysine methyl ester dihydrochloride

sodium hydroxide

→

L-lysine

Растворители

methanol

Условия реакции

Обработка

1. 1
   Другоеto prepare a treating agent solution
2. 2
   Другоеto uniformly absorb the total amount of the above treating agent solution
3. 3
   Другоеair-dried for an hour at room temperature
4. 4
   workup.ADDITIONThe dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.)
5. 5
   Промывкаwashed with water
6. 6
   ПромывкаIt was further washed with 5% aqueous sodium bicarbonate solution
7. 7
   Промывкаrinsed three times with water
8. 8
   Промывкаsubsequently was washed with 0.05 N aqueous hydrochloric acid solution
9. 9
   Промывкаrinsed three times with water
10. 10
    Промывкаfinally washed with a JAFET standard detergent solution for household
11. 11
    Промывкаwashing machine
12. 12
    Промывкаrinsed with water
13. 13
    Другоеair-dried

Методика

1.16 Gram (5 mmol) of L-lysine methyl ester dihydrochloride was dissolved in 4.5 ml of methanol, and 2.5 ml of 2 N aqueous sodium hydroxide solution was added thereto to prepare a treating agent solution. An untreated cotton cloth (160 mm×170 mm, 5.0 g) was allowed to uniformly absorb the total amount of the above treating agent solution and then air-dried for an hour at room temperature. The dried cloth was heat-treated for 20 minutes at 140° C. in a batch type hot air oven (“IPHH-2000”, an inert oven manufactured by Tabaiesbeck Co., Ltd.) and then washed with water. It was further washed with 5% aqueous sodium bicarbonate solution and rinsed three times with water and subsequently was washed with 0.05 N aqueous hydrochloric acid solution and rinsed three times with water, and finally washed with a JAFET standard detergent solution for household washing machine, rinsed with water and air-dried. A part of this cloth was cut and a solution of ninhydrin in butanol was sprayed on it. Thereafter, it was heated for 10 minutes in an oven of 110° C. to cause coloring thereby the presence of the amino acid was confirmed. Also, a part of the cloth was cut and subjected to an infrared spectroscopic analysis whereby there was confirmed a peak based on ester bond at 1749 cm−1. As a result of determination for the amount of the bonded L-lysine, it was 0.10 mmol based on 1 g of the cloth. The degree of esterfication was 0.016.

Источник