Реакция #8611

Уравнение реакции

EGCG

H2O2

→

theaflavin 3-gallate

Выход 28.1%

Растворители

acetone

Условия реакции

Обработка

1. 1
   ЭкстракцияThe reaction mixture was extracted by ethyl acetate (50 ml×3)
2. 2
   КонцентрированиеAfter concentration
3. 3
   Промывкаeluted with acetone-water solvent system (45%)

Методика

EC (1 g) and EGCG (1 g) were dissolved in a mixture of acetone-pH 5.0 phosphate citrate buffer (1:10, v/v, 50 mL), which contained 4 mg horseradish peroxidase. While being stirred, 2.0 ml of 3.13% H2O2 was added four times during 45 minutes. The reaction mixture was extracted by ethyl acetate (50 ml×3). After concentration, the residue was subjected to Sephadex LH 20 column eluted with acetone-water solvent system (45%). 220 mg theaflavin 3-gallate was obtained.

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