Реакция #82860

ord-1825b608ebc244d3b221ee45bb66642a

Уравнение реакции

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

→

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA DOPC

Реактанты

$CCCCCCCC/C=C\CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C\CCCCCCCC.[Cl-]$

DOTMA

$CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)([O-])OCC[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC$

DOPC

Реагенты

Нет

Растворители

chloroform

Условия реакции

Подробные условия

See reaction.notes.procedure_details.

Обработка

1
ДругоеDOTMA/DOPC vesicles were prepared
2
Другоеremoving the solvent
3
Другоеby drying under a stream of nitrogen gas
4
Другоеwas placed under vacuum overnight
5
Другоеto remove traces of solvent
6
Другоеsonicated in a bath type sonicator for 30 minutes

Методика

DOTMA/DOPC vesicles were prepared by dissolving 10 mg DOTMA and 10 mg DOPC in chloroform and removing the solvent by drying under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 1 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear producing DOTMA/DOPC SUV's at a concentration of 20 mg/ml total lipid.

Источник

DOI: 10.6084/m9.figshare.5104873.v1Патент: US05622712uspto-grants-1997_04