Реакция #806254

Уравнение реакции

ethyl (S)-4-chloro3-hydroxybutyrate

NaCN

→

ethyl (R)-4-cyano-3-hydroxybutyrate

Растворители

potassium phosphate

Условия реакции

Обработка

1. 1
   ПромывкаAfter fifty seven hours the mixture was washed with ethyl acetate (2 times 250 mL)
2. 2
   Сушкаthe combined organics dried over anhydrous sodium sulfate
3. 3
   ФильтрацияThe mixture was filtered
4. 4
   Другоеevaporated on a rotary evaporator

Методика

To a well stirred solution of ethyl (S)-4-chloro3-hydroxybutyrate (8.25 g, 50 mmoles) in 300 mM potassium phosphate buffer, 300 mM NaCN pH 8.0 (1 L) at 30° C. was added halohydrin dehalogenase SEQ ID NO: 14 (9 g) as a lyophilized powder. After fifty seven hours the mixture was washed with ethyl acetate (2 times 250 mL) and the combined organics dried over anhydrous sodium sulfate. The mixture was filtered and evaporated on a rotary evaporator to give essentially pure ethyl (R)-4-cyano-3-hydroxybutyrate, as determined using the gas chromatography method and elution time data described in Example 6, hereinbelow.

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