Реакция #69297

Уравнение реакции

tert-Butyl 4-(cyanomethyl)-4-[4-(7-[2-(trimethylsilyl)ethoxy]methyl-7H-pyrrolo[2,3-d]-pyrimidin-4-yl)-1H-pyrazol-1-yl]piperidine-1-carboxylate

TFA

→

product

Выход 3.6%

4-[4-(7H-Pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl]piperidin-4-ylacetonitrile

Выход 3.6%

Условия реакции

Обработка

1. 1
   КонцентрированиеThe solution was concentrated
2. 2
   Другоеto remove the TFA
3. 3
   workup.ADDITIONMethanol was added to the resulting residue
4. 4
   Концентрированиеthe resulting mixture was concentrated
5. 5
   workup.DISSOLUTIONThe resulting residue was dissolved in methanol (10 mL)
6. 6
   workup.ADDITION15.0 M ammonium hydroxide in water (1.66 mL) was added
7. 7
   workup.STIRRINGThe resulting solution was stirred for 2 h
8. 8
   КонцентрированиеThe mixture was concentrated
9. 9
   workup.ADDITIONToluene was added to the resulting residue
10. 10
    Концентрированиеthe resulting mixture was concentrated
11. 11
    Другоеto provide a white semisolid
12. 12
    ДругоеThe rest was purified by prep HPLC
13. 13
    workup.WAIT8% ACN—H2O (0.1% NH4OH), 1.0 min, to 27% at 6 min
14. 14
    Другоеretention time, 5.4 min
15. 15
    workup.ADDITIONTubes containing pure product
16. 16
    Другоеfreeze dried

Методика

tert-Butyl 4-(cyanomethyl)-4-[4-(7-[2-(trimethylsilyl)ethoxy]methyl-7H-pyrrolo[2,3-d]-pyrimidin-4-yl)-1H-pyrazol-1-yl]piperidine-1-carboxylate (0.670 g, 1.24 mmol) was dissolved in TFA (5.0 mL, 65 mmol) and was stirred for 1.3 h. LCMS showed conversion to the hydroxymethyl intermediate, M+H 338. The solution was concentrated to remove the TFA. Methanol was added to the resulting residue, and the resulting mixture was concentrated. The resulting residue was dissolved in methanol (10 mL) and 15.0 M ammonium hydroxide in water (1.66 mL) was added. The resulting solution was stirred for 2 h. LCMS and HPLC analyses showed complete deprotection. The mixture was concentrated. Toluene was added to the resulting residue and the resulting mixture was concentrated to provide a white semisolid. Most of this intermediate product was used for the next step. The rest was purified by prep HPLC using a 30 mm×100 mm C18 column; 8% ACN—H2O (0.1% NH4OH), 1.0 min, to 27% at 6 min; 60 mL/min; detector set at m/z 308; retention time, 5.4 min. Tubes containing pure product were combined and freeze dried to give 13.6 mg of the product.

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