Реакция #324426

Уравнение реакции

HCl methanol

Pulchinenoside A

→

desired product

Выход 85.0%

3,23-Dihydroxy-20(29)-lupen-28-oic acid

Выход 85.0%

Растворители

water

Условия реакции

Обработка

1. 1
   Экстракцияthe mixture was extracted with chloroform (3×50 ml)
2. 2
   ПромывкаThe combined organic layer was washed with H2O (1×10 ml), brine (1×10 ml)
3. 3
   Сушкаdried over sodium sulfate
4. 4
   Концентрированиеconcentrated under vacuum
5. 5
   ДругоеThe resulting residue was purified by column chromatography on silica gel

Методика

To a solution of HCl methanol reagent 10 (10 mL) was added Pulchinenoside A (DA001, 100 mg) and the resulting mixture was stirred at 35° C. for 4 days. After pouring into water (10 ml), the mixture was extracted with chloroform (3×50 ml). The combined organic layer was washed with H2O (1×10 ml), brine (1×10 ml), dried over sodium sulfate and concentrated under vacuum. The resulting residue was purified by column chromatography on silica gel using CH2Cl2-MeOH (v/v, 30:1) to yield the desired product as a white solid with 85% yield.

Источник