Реакция #2782

ord-56ee7e03a5e84bf7aaab24c26d7d4d75

Уравнение реакции

CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O
N-acetyl-D-glucosamine
O=P([O-])([O-])[O-]
phosphate
→
N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O
D-glucosamine

Реактанты

—
enzyme solution
—
solution
CC(=O)N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O
N-acetyl-D-glucosamine
O=P([O-])([O-])[O-]
phosphate

Реагенты

Нет

Условия реакции

Подробные условия
See reaction.notes.procedure_details.

Методика

The activity is determined by the following method. Namely, 0.3 ml of a 10% solution of N-acetyl-D-glucosamine is added as a substrate to 0.1 ml of a 200 mM phosphate buffer solution (pH 7.8). Next, 0.1 ml of an enzyme solution is further added thereto followed by incubation at 48° C. for 30 minutes. Then the D-glucosamine thus formed is determined by the indole/hydrochloric acid colorimetry. The amount of the enzyme by which 1 μmol of D-glucosamine is formed in a minute is referred to as 1 U (unit).

Источник

DOI: 10.6084/m9.figshare.5104873.v1Патент: US05731184uspto-grants-1998_03