Реакция #216577

Уравнение реакции

product

N-4-chlorobutanoylvalyl-isoleucyl-methionine methyl ester

NaOH

→

N-[2(S)-(pyrrolidin-2-on-1-yl)-3-methylbutanoyl]-isoleucyl-methionine

Растворители

methanol

Условия реакции

Обработка

1. 1
   ДругоеAfter 1 h. the methanol was evaporated
2. 2
   workup.DISSOLUTIONthe residue was dissolved in water
3. 3
   Фильтрацияfiltered
4. 4
   Экстракцияthe product was extracted into ethyl acetate
5. 5
   ДругоеAfter standard workup, a solid was obtained
6. 6
   Промывкаwhich was washed with water and ether
7. 7
   ДругоеAfter drying the solid

Методика

The product of Step A was suspended in 5 mL of methanol and 0.6 mL of 1N NaOH was added. After 1 h. the methanol was evaporated, the residue was dissolved in water and filtered. The filtrate was acidified and the product was extracted into ethyl acetate. After standard workup, a solid was obtained, which was washed with water and ether. After drying the solid weighed 25 mg. FAB mass spectrum m/z=430 (M+1).

Источник