Реакция #2045076

Уравнение реакции

NaOH

xylose

furfural

→

xylitol

Условия реакции

Обработка

1. 1
   Другоеobtained by a pretreatment process of acid-catalyzed steam explosion
2. 2
   Другоеis 180° C.
3. 3
   workup.ADDITIONThe reaction mix
4. 4
   Другоеis then separated by a solid-liquid separation equipment
5. 5
   Другоеto get the liquid fraction as hydrolysate
6. 6
   Другоеis controlled at 30° C.
7. 7
   Температураthe agitation of the incubator is maintained at 100 rpm
8. 8
   workup.WAITafter 40 hr of fermentation

Методика

The fermentation medium is napiergrass hydrolysate obtained by a pretreatment process of acid-catalyzed steam explosion. Pretreatment conditions: the concentration of the dilute acid is 2%, the operation temperature is 180° C., the reaction time at this high temperature is 5 min, after which the reactor is immediately depressurized. The reaction mix is then separated by a solid-liquid separation equipment to get the liquid fraction as hydrolysate. In this case, NaOH is added into the napiergrass hydrolysate to adjust to pH 6.0, the fermentation temperature is controlled at 30° C., the agitation of the incubator is maintained at 100 rpm, the ratio of the inocula size of the culture and the volume of the fermentation medium is 1:6 (v/v). The content of xylose in the napiergrass hydrolysate is 15.55 g/L, the content of the inhibitor furfural is 1.19 g/L, and after 40 hr of fermentation, the amount of xylitol produced is 5.11 g/L, and the overall utilization of xylose is up to 95.2%, as shown in Table 5.

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