Реакция #2041
ord-48e5492592614940b5cbab975adec0bb
Уравнение реакции
calcium chloride
sodium pyrophosphate
calcium phosphorus
→
calcium diphosphate dihydrate
Реагенты
Нет
Условия реакции
Подробные условия
See reaction.notes.procedure_details.
Обработка
- 1workup.WAITto stand for a whole day
Методика
An aqueous calcium chloride solution and an aqueous sodium pyrophosphate solution were mixed together so that the calcium/phosphorus atomic ratio became 1:1, and the mixture was allowed to stand for a whole day to form calcium diphosphate dihydrate (Ca2P2O7.2H2O) crystals, which were then filtered off. The thus prepared material was packed into a column in the same manner as in Example 1, and a mixed sample of serum albumin, lysozyme and cytochrome c was chromatographed thereon. As a result the serum albumin flowed through the column without being retained therein, while the lysozyme and cytochrome c were retained therein.