Реакция #2028924

Уравнение реакции

L-fructose

L-glucose

→

6-deoxy L-glucose

6-deoxy L-fructose

Условия реакции

Методика

The present inventors already publicly report that K. pneumonia 40bXX catalyzes the conversion from L-fructose to L-glucose. By the same method, attempts were made to produce 6-deoxy L-glucose from 6-deoxy L-fructose (the product at the previous step). K. pneumonia 40bXX was cultured in a culture medium containing 0.5 w/v % polypeptone, 0.5 w/v % yeast extract, and 0.5 w/v % salt as supplemented with MnCl2 (1 mM). After 24-hour incubation, the culture was centrifuged at 1200×g for 10 minutes, to recover the bacterial cells. The recovered bacterial cells were washed twice with 50 mM glycine-NaOH buffer (pH 9.0), and then centrifuged at 1200×g for 10 minutes. The washed bacterial cells were disrupted with a motor, to which activated alumina was added for grinding the bacterial cells to recover a crude enzyme. The partially purified enzyme D-AI (400 U) is preliminarily adjusted with 50 mM glycine-NaOH, and then immobilized on 30 g (wet weight) of Chitopearl BCW 2510 for use in the reaction. The rearrangement reaction was progressed in an L-tube containing 1% 6-deoxy L-fructose, the immobilized D-AI (40 U), 50 mM Tris-HCl buffer (pH 9.0) and MnCl2 (to a final concentration: 1 mM) at 40° C.

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