Реакция #2006456

Уравнение реакции

sodium hydroxide

1-(4-methoxyphenyl)-2-propanone

D-glucose

NAD+

D-alanine

pyridoxal phosphate

→

1-(4-methoxyphenyl)-2-aminopropane

Условия реакции

Обработка

1. 1
   Другое(pNTASPAG), obtained in Example 11, which
2. 2
   workup.ADDITIONwere added
3. 3
   ДругоеDuring the reaction
4. 4
   Другоеthe reaction liquid
5. 5
   workup.ALIQUOTwas sampled
6. 6
   ЭкстракцияFrom the sample, 1-(4-methoxyphenyl)-2-aminopropane was extracted by the addition of ethyl acetate

Методика

To a flask containing 1.5 g of a substrate 1-(4-methoxyphenyl)-2-propanone, 4.94 g of D-glucose, 6.1 mg of NAD+, 4.88 g of D-alanine, and 4.0 mg of pyridoxal phosphate, 30 ml of a culture fluid of the E. coli HB101 (pNTASPAG), obtained in Example 11, which expresses TAS, PALDH, and GDH were added. The resultant product was stirred at 30° C. while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. During the reaction, the reaction liquid was sampled. From the sample, 1-(4-methoxyphenyl)-2-aminopropane was extracted by the addition of ethyl acetate after basifying the sample with 6 N aqueous solution of sodium hydroxide. The amount of 1-(4-methoxyphenyl)-2-aminopropane produced was measured by analyzing 1-(4-methoxyphenyl)-2-aminopropane under the following HPLC conditions. Further, by the law of the art, 1-(4-methoxyphenyl)-2-aminopropane thus obtained was acted on by 3,5-dinitrobenzyl chloride to form a dinitrobenzyl derivative. After that, the dinitrobenzyl derivative was analyzed under the following HPLC conditions, whereby the optical purity thereof was measured.

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