Реакция #1951041

Уравнение реакции

ammonia

Compound 7

3′,5′-di-O-acetyl-4′-C-cyano-2′-deoxy-2-fluoroadenosine

→

4′-C-cyano-2′-deoxy-2-fluoroadenosine

Растворители

methanol

Условия реакции

Обработка

1. 1
   ДругоеThe resultant reaction mixture
2. 2
   Концентрированиеwas concentrated under reduced pressure
3. 3
   Другоеthe resultant residue was purified by means of silica gel column chromatography (silica gel 10 ml, hexane/ethyl acetate (5:1), ethyl acetate, ethyl acetate/methanol (10:1))

Методика

Compound 7 (53.7 mg, 0.142 mmol) was dissolved in methanol (1.90 ml), and 28% aqueous ammonia (1.30 ml) was added to the resultant solution, followed by stirring at room temperature for 30 minutes. The resultant reaction mixture was concentrated under reduced pressure, and then the resultant residue was purified by means of silica gel column chromatography (silica gel 10 ml, hexane/ethyl acetate (5:1), ethyl acetate, ethyl acetate/methanol (10:1)), to thereby yield compound 8 (30.2 mg, 0.10 mmol, 72.3%).

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