Реакция #1951024

Уравнение реакции

2,5,6-Trichloro-3-formyl-1-(β-D-ribofuranosyl)indole

methyl hydrazinocarboxylate

water

→

4.31

Выход 85.0%

2,5,6-Trichloro-3-[N-(methoxycarbonylamino)iminomethylidene]-1-(β-D-ribofuranosyl)indole

Выход 85.0%

Растворители

MeOH

Условия реакции

Обработка

1. 1
   ТемператураThe resulting solution was heated on a 60 C oil bath for 16 h
2. 2
   Другоеthe solvent evaporated to approx 50 mL
3. 3
   ТемператураThe resulting suspension was cooled to 4 C
4. 4
   Фильтрацияfiltered
5. 5
   Промывкаthe solids rinsed with cold water (25 mL)
6. 6
   ДругоеThe solids were recrystallized

Методика

2,5,6-Trichloro-3-formyl-1-(β-D-ribofuranosyl)indole (4.3, 122 mg, 0.32 mmol) was dissolved in MeOH (10 mL) to which was added methyl hydrazinocarboxylate (115 mg, 1.3 mmol). The resulting solution was heated on a 60 C oil bath for 16 h, then cooled to room temperature and poured into 100 mL of water, and the solvent evaporated to approx 50 mL. The resulting suspension was cooled to 4 C, then filtered and the solids rinsed with cold water (25 mL). The solids were recrystallized from boiling EtOAc/hexane to yield 123 mg (85%) of 4.31 as a pale pink crystalline solid: mp 217-219 C; Rf0.2 (10% MeOH/CHCl3); 1H-NMR (500 MHz, DMSO-d6): δ 11.17 (b, 1H, D2O exch.), 8.54 (s, 1H), 8.42 (s, 1H), 8.22 (b, 1H), 5.92 (d, 1H), 5.42-5.37 (m, 2H, D2O exch.), 5.25 (d, 1H, D2O exch.), 4.43 (q, 1H), 4.15 (d, 1H), 3.98 (d, 1H), 3.72 (b, 5H). 13C-NMR (125 MHz, DMSO-d6): δ 153.88, 133.17, 129.33, 126.08, 124.96, 124.06, 122.17, 115.34, 108.59, 88.76, 86.03, 71.30, 69.73, 69.62, 61.07, 52.04. HRMS (ES) m/z calcd. for C16H16Cl3N3O6.Na 474.0002, found 473.9999. Anal calcd for C16H16Cl3N3O6.¼H2O: C, 42.03; H, 3.64; N, 9.19. Found: C, 42.11; H, 3.92; N, 9.18.

Источник