Реакция #1435708

Уравнение реакции

maltotriose

→

maltose

Растворители

acetate

Условия реакции

Обработка

1. 1
   ДругоеThe activity of α-isomaltosylglucosaccharide-forming enzyme
2. 2
   ДругоеA substrate solution was prepared
3. 3
   Другоеto give a concentration of 2% (w/v)
4. 4
   ДругоеA reaction mixture was prepared
5. 5
   Другоеthe reaction
6. 6
   workup.WAITby boiling for 10 minutes

Методика

The two types of enzymatic activities described above were measured as following. The activity of α-isomaltosylglucosaccharide-forming enzyme was measured by the following assay: A substrate solution was prepared by dissolving maltotriose in 100 mM acetate buffer (pH 6.0) to give a concentration of 2% (w/v). A reaction mixture was prepared by mixing 0.5 ml of the substrate solution and 0.5 ml of an enzyme solution, and incubated at 35° C. for 60 minutes. After stopping the reaction by boiling for 10 minutes, the amount of maltose formed in the reaction mixture was determined by high-performance liquid chromatography (HPLC). One unit of α-isomaltosylglucosaccharide-forming activity was defined as the amount of the enzyme that forms one μmole of maltose per minute under the above conditions. HPLC was carried out using “SHODEX KS-801 column”, Showa Denko K. K., Tokyo, Japan, at a column temperature of 60° C. and a flow rate of 0.5 ml/minutes of water, and using “RI-8012”, a differential refractometer commercialized by Tosoh Corporation, Tokyo, Japan.

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