반응 #949032

반응 방정식

tert-butyl (2RS,4SR,5RS)-1-{2-[3-(3-methoxycarbonylmethylphenyl)ureido]acetyl}-5-(2-fluorophenyl)-4-(4-chlorophenyl)sulphonyl-2-pyrrolidinecarboxylate

potassium hydroxide

→

(2RS,4SR,5RS)-3-(3-{2-[2-tert-butoxycarbonyl-5-(2-fluorophenyl)-4-(4-chlorophenyl)sulphonyl-1-pyrrolidinyl]-2-oxoethyl}ureido)phenylacetic acid

수율 38.8%

용매

methanol

water

반응 조건

후처리

1. 1
   농축concentrated under reduced pressure
2. 2
   세척washed with three times 75 cm3 of diethyl ether
3. 3
   추출extracted with three times 75 cm3 of ethyl acetate
4. 4
   세척washed with three times 50 cm3 of saturated aqueous sodium chloride solution
5. 5
   건조dried over magnesium sulphate
6. 6
   농축concentrated under reduced pressure
7. 7
   기타The residue is purified by chromatography on 80 g of silica
8. 8
   workup.ADDITIONThe fractions containing the expected product
9. 9
   농축concentrated under reduced pressure

실험 절차

A To a solution of 2.5 g of tert-butyl (2RS,4SR,5RS)-1-{2-[3-(3-methoxycarbonylmethylphenyl)ureido]acetyl}-5-(2-fluorophenyl)-4-(4-chlorophenyl)sulphonyl-2-pyrrolidinecarboxylate in 60 cm3 of methanol and 30 cm3 of distilled water is added, at a temperature in the region of 20° C., 0.2 g of potassium hydroxide. The reaction mixture is stirred for twenty hours at a temperature in the region of 20° C., and then concentrated under reduced pressure. The residue is taken up in 100 cm3 of distilled water, washed with three times 75 cm3 of diethyl ether, acidified to a pH in the region of 1 by aqueous normal hydrochloric acid and extracted with three times 75 cm3 of ethyl acetate. The extracts are combined, washed with three times 50 cm3 of saturated aqueous sodium chloride solution, dried over magnesium sulphate and concentrated under reduced pressure. The residue is purified by chromatography on 80 g of silica contained in a column 3.2 cm in diameter [eluent: dichloromethane/methanol (95/5 by volume)]. The fractions containing the expected product are combined and concentrated under reduced pressure. 0.95 g of (2RS,4SR,5RS)-3-(3-{2-[2-tert-butoxycarbonyl-5-(2-fluorophenyl)-4-(4-chlorophenyl)sulphonyl-1-pyrrolidinyl]-2-oxoethyl}ureido)phenylacetic acid is thus obtained [Rf=0.5; eluent: methylene chloride/methanol (90/10); 1H NMR spectrum (250 MHz, (CH3)2SO with addition of a few drops of CD3COOD, at a temperature of 393 K, δ in ppm): 1.54 (s, 9H: C(CH3)3); from 2.40 to 2.60 and 2.85 (2 mts, 1H each: CH2 at 3); 3.50 (s, 2H: ArCH2COO); 3.70 and 3.97 (broad d and d respectively, J=17 Hz, 1H each: COCH2N); 4.09 (mt, 1H: H4); 4.68 (t, J=8 Hz, 1H: H2); 5.73 (d, J=3 Hz, 1H: H5); 6.86 (broad d, J=7.5 Hz, 1H: aromatic H (H4: ortho to the CH2)); from 7.00 to 7.40 (mt, 6H: aromatic H); 7.68 (broad d, J=8.5 Hz, 2H: H of the aromatic at 4 (H ortho to the Cl)); 7.82 (broad t, J=8 Hz, 1H: H of the aromatic at 5 (H6)); 7.96 (broad d, J=8.5 Hz, 2H: aromatic H at 4 (H meta to the Cl))].

출처