반응 #94145

반응 방정식

Nα -(2-acetylthiomethyl-5-aminopentanoyl)-L-arginine trifluoroacetate

→

Nα -(5-Amino-2-mercaptomethylpentanoyl)-L-arginine

용매

water

ammonia

반응 조건

후처리

1. 1
   농축The solution is concentrated in vacuo to ca. 5 ml
2. 2
   세척After washing with water Nα -(5-amino-2-mercaptomethylpentanoyl)-L-arginine
3. 3
   세척is eluted with a pyridine-acetate buffer at pH 6.5

실험 절차

Nα -(2-acetylthiomethyl-5-aminopentanoyl)-L-arginine trifluoroacetate (2 g) is dissolved in a mixture of water (25 ml) and concentrated ammonia (25 ml) and this solution is stored at room temperature for 20 minutes. The solution is concentrated in vacuo to ca. 5 ml and applied to a column of Dowex 50 ion exchange resin (50 ml) in the hydrogen cycle. After washing with water Nα -(5-amino-2-mercaptomethylpentanoyl)-L-arginine is eluted with a pyridine-acetate buffer at pH 6.5.

출처