반응 #8546

반응 방정식

Methyl 2-(2,3,4-trifluoroanilino)propionate

methyl (2R)-2-(2,3,4-trifluoroanilino)propionate

→

title compound

수율 47.9%

(2S)-2-(2,3,4-Trifluoroanilino)propionic acid

수율 47.9%

용매

phosphate

반응 조건

후처리

1. 1
   workup.ADDITIONThen the above-described liquid culture (10 ml) was added
2. 2
   workup.STIRRINGThe mixture was stirred for additional 16 hours

실험 절차

Microbial cells (IFO-1575; Zygoascus hellenicus) were cultured in an MY medium (pH 6.0; 50 ml) at 30° C. for 48 hours. Methyl 2-(2,3,4-trifluoroanilino)propionate (1.0 g) was suspended in a 0.1 M phosphate buffer solution (pH 6.5; 90 ml). Then the above-described liquid culture (10 ml) was added thereto and gently stirred. The mixture was stirred for additional 16 hours while maintaining at 30° C. Then it was treated as in Example 43 to thereby give methyl (2R)-2-(2,3,4-trifluoroanilino)propionate (0.39 g, optical purity 91% ee) and the title compound (0.45 g, optical purity 84% ee).

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